Laboratory Manual for Biomedical Research
New Edition
Chapter 1. General Lab Techniques
Lab security and basic techniques
Chapter 2. Molecular Biology -- Molecular Separation
DNA Agarose Gel Electrophoresis
RNA Agarose Gel Electrophoresis
SDS-Page - Polyacrylamide gel electrophoresis
2D Page-Two - Dimensional Polyacrylamide Gel Electrophoresis
Chapter 3. Molecular Biology -- DNA and RNA
DNA isolation & related protocols
DNA Purification (glass milk vs electroelution)
RNA Isolation and Purification
Isolation of DNA,RNA, and Protein simultaneously.
DNA mutation detection by SSCP
Preparation of DNA and RNA probes
Basic knowledge of microarray.
MicroArray Procedure
Total RNA Isolation from cultured cells.
Making the single strand cDNA probe.
Automated Slide Processor (ASP) Version for hybridization.
Pre-hybridization of the processed slides (NON-Automated version).
Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).
Preparation of Dendrimer Cy3 and Cy5.
Washing unbound probe from glass array (NON-Automated version).
Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).
Washing unbound dendrimer from glass array(NON-Automated version).
Other Microarray Protocols (1,2)
Conditional gene transfection(Tet on/off)
Chapter 4. Genetics
Single nucleotide polymorphisms (SNPs)
Chapter 5. Molecular Biology --PCR Serials
PCR (General Procedure)
Recommended Reagent Concentrations
Recommended Reaction Conditions
Asymmetric PCR for ssDNA Production
Labelling PCR Products with Digoxigenin
Chapter 6. Molecular Biology – Protein
Western Blot Hybridization Center
Western Blot Full Procedure (from Pierce)
Western Blotting Procedure with Video Show
Protocol 1: Western Blot
Incubation with primary antibody
Incubation with secondary antibody
Restore a western blot using pierce stripping buffer
Western blot related solutions and buffers
Protocol 2: Western Blot 2.
Protocol 3: Western Blot 3.
More online Western blot protocols (1) (2)
Methods for detecting protein phosphorylation
Chapter 7. DNA Protein Interactions
Chapter 8. Immunohistochemistry/immunology
Immunology/Histology
Preparing silanized (+plus) slides
Special cell & cell fraction stains
Conjugation of monoclonal antibodies
Immunoperoxidase staining techniques
Histotechnology--technical methods
Chapter 9. Cellular Biology
General cell culture protocols
Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
What are the unique properties of all stem cells?
What are embryonic stem cells?
What are the similarities and differences between embryonic and adult stem cells?
Where can I get more information?
International Society for Stem Cell Research (ISSCR)
Culture of Human Embryonic Stem Cells (hESC)
Establishment and characterization of human embryonic stem cell line
Rat stem cell isolation and culture
Mouse stem cell isolation and culture
Induction of Stem Cell Differentiation
Cancer stem cells
Video Data on Stem Cell Research (click to show)
Apoptosis and related protocols
Soft Agar Assay for Colony Formation
Blood cell fractionation (white blood cell isolation)
Endothelial cell isolation and culture protocols
Chapter 10. Metabolism, GC/MS, NMR and Proteomics
Glucose metabolism and its related protocols
Lactate cleanup and derivative
Isotope Ratio Mass Spectrometer (IRMS)
Liquid chromatography / mass spectrometer (LC/MS)
Chapter 11. Animal Experiments
Basic skills for animal experiments
Anatomy and physiology of animals
Cancer xenograft animal models
Animal models for depression-like and anxiety-like behavior
Chapter 12. Worm: C. Elegans
Chapter 13. HPLC and TLC
Chapter 14. Buffers and Solutions in Lab.
Commonly Used Buffer and solution (114 formats in alphabeta)
Compound solutions (Practical Molecular Biology)
How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)
Reto's Buffers&Media Book (Private Page)
Salt solutions(Practical Molecular Biology)
Single Component Solutions (Practical Molecular Biology)
Buffers (Gerard R. Lazo)
Antibiotics (Gerard R. Lazo)
Buffer Listing (Salmon Lab.)
Cell Culture Media and Solutions (Donis-Keller lab)
Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )
Commonly used solutions (2)--- 1 ) 2 ) 3 )
Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )
Chapter 15. Other Resources
Cinema Online, Free Movies --(1) (2) (3)
Progress in Cancer Apoptosis -- (1) (2) (3)
Progress in Gene Therapy --- (1) (2) (3)
Progress in Neuronal Regeneration -- (1) (2) (3)
Progress in AIDS Treatment -- (1) (2) (3)
Progress in Organ Transplantation -- (1) (2) (3)
Progress in Stem Cell Research -- (1) (2) (3)
Free eBooks in life science (1)
Free eBooks in life science (2,3)
Free Software pDRAW32 to Draw DNA Analysis Charts
Biological Educational Resources
Resources of Medical Biochemistry
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