Laboratory Manual for Biomedical Research

New Edition

Chapter 1. General Lab Techniques

Lab security and basic techniques

Advanced lab skills

Advanced lab skills (2)

Chapter 2. Molecular Biology -- Molecular Separation

Molecular Separation

DNA Agarose Gel Electrophoresis

RNA Agarose Gel Electrophoresis

SDS-Page - Polyacrylamide gel electrophoresis

2D Page-Two - Dimensional Polyacrylamide Gel Electrophoresis

Ion Exchange Chromatography

Gel Filtration Chromatography

Affinity chromatography

Chapter 3. Molecular Biology -- DNA and RNA

Nucleic acid methods (1)

Nucleic acid methods (2)

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA, RNA Sequencing

RNA Isolation and Purification

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Preparation of DNA and RNA probes

Southern blot hybridization

Northern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

SiRNA gene knockout

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Basic knowledge of microarray.

Introduction to Microarray.

MicroArray Procedure

Total RNA Isolation from cultured cells.

DNase Treatment of Total RNA

Making the single strand cDNA probe.

Automated Slide Processor (ASP) Version for hybridization.

Washing microarrays in ASP.

Processing of Array slide

Pre-hybridization of the processed slides (NON-Automated version).

Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).

Preparation of Dendrimer Cy3 and Cy5.

Washing unbound probe from glass array (NON-Automated version).

Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).

Washing unbound dendrimer from glass array(NON-Automated version).

Microarray Dababases

Troubleshooting

Other Microarray Protocols (1,2)

Gene transfection

Gene therapy for cancer

Molecular cloning

Conditional gene transfection(Tet on/off)

Chapter 4. Genetics

Epigenetics protocols

Mutagenesis protocols

Single nucleotide polymorphisms (SNPs)

Chapter 5. Molecular Biology --PCR Serials

PCR,RT-PCR,Real time PCR etc.

PCR (General Procedure)

Recommended Reagent Concentrations

Recommended Reaction Conditions

Initial Conditions

Temperature Cycling

"Hot Start" PCR

Asymmetric PCR for ssDNA Production

Detecting Products

Labelling PCR Products with Digoxigenin

Cleaning PCR Products

Sequencing PCR Products

Cloning PCR Products

AND ALWAYS REMEMBER

PCR Primer Design Tools

RT-PCR

Real time PCR

More PCR Protocols Online

Video and Animation of PCR

Mouse Genotyping by PCR

PCR Based Molecular Cloning

PCR Troubleshootings

Chapter 6. Molecular Biology – Protein

Protein methods

Protein labeling techniques

Protein sequencing

Subcellular fractionations

Western blot hybridization

Western Blot Hybridization Center

Western Blot Full Procedure (from Pierce)

Western Blotting Procedure with Video Show

Protocol 1: Western Blot

Sample protein preparation

Protein concentration assay

Electrophoresis and blotting

Blocking non-specific antigen

Incubation with primary antibody

Incubation with secondary antibody

Substrate (ECL) incubation

Restore a western blot using pierce stripping buffer

Western blot related solutions and buffers

Western blot handbook

Western blot troubleshooting

Protocol 2: Western Blot 2.

Protocol 3: Western Blot 3.

More online Western blot protocols (1) (2)

Protein chips

Kinase assay

Methods for detecting protein phosphorylation

Chapter 7. DNA Protein Interactions

EMSA

ChIP assay

Chapter 8. Immunohistochemistry/immunology

Immunology/Histology

Preparing silanized (+plus) slides

Microscopy Techniques

Confocal microscope technique

Electron microscopy

HE staining

DAPI & PI nucleic acid stain

Hybridization in situ

FISH

Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Immunoperoxidase staining techniques

Immunofluorohistochemistry

Immunoprecipitation

Histotechnology--technical methods

FRQs for histochemistry

Laser Capture Microdissection

Elisa

Chapter 9. Cellular Biology

General cell culture protocols

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Introduction

What are the unique properties of all stem cells?

What are embryonic stem cells?

What are adult stem cells?

What are the similarities and differences between embryonic and adult stem cells?

What are the potential uses of human stem cells and the obstacles that must be overcome before these potential uses will be realized?

Where can I get more information?

International Society for Stem Cell Research (ISSCR)

Culture of Human Embryonic Stem Cells (hESC)

Establishment and characterization of human embryonic stem cell line

Rat stem cell isolation and culture

Mouse stem cell isolation and culture

Induction of Stem Cell Differentiation

Cancer stem cells

Video Data on Stem Cell Research (click to show)

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Blood cell fractionation (white blood cell isolation)

Endothelial cell isolation and culture protocols

Flow cytometry (FCM)

Chapter 10. Metabolism, GC/MS, NMR and Proteomics

GC/MS Background

Glucose metabolism and its related protocols

PAS staining

Deoxyribose procedure

Ribose metabolism analysis

Polysaccharide sequencing

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols

Cholesterol

Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)

Proteomics

NMR protocols and tutorials

Chapter 11. Animal Experiments

Blood sampling from animals

Basic skills for animal experiments

Anatomy and physiology of animals

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Animal models for depression-like and anxiety-like behavior

Chapter 12. Worm: C. Elegans

C. Elegans Protocols

Chapter 13. HPLC and TLC

HPLC protocols

TLC

Chapter 14. Buffers and Solutions in Lab.

Commonly Used Buffer and solution (114 formats in alphabeta)

Compound solutions (Practical Molecular Biology)
How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)
Reto's Buffers&Media Book (Private Page)
Salt solutions(Practical Molecular Biology)
Single Component Solutions (Practical Molecular Biology)
Buffers (Gerard R. Lazo)

Antibiotics (Gerard R. Lazo)
Buffer Listing (Salmon Lab.)

Cell Culture Media and Solutions (Donis-Keller lab)

Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )

Commonly used solutions (2)--- 1 ) 2 ) 3 )

Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )

Chapter 15. Other Resources

Free eBooks at Library Online

Cinema Online, Free Movies --(1) (2) (3)

Progresses in Life Science

Progress in Cancer Apoptosis -- (1) (2) (3)

Progress in Gene Therapy --- (1) (2) (3)

Progress in Neuronal Regeneration -- (1) (2) (3)

Progress in AIDS Treatment -- (1) (2) (3)

Progress in Organ Transplantation -- (1) (2) (3)

Progress in Stem Cell Research -- (1) (2) (3)

Free eBooks in life science (1)

Free eBooks in life science (2,3)

Most Updated Biomedical Books

Pathway databases

Pathway Search

Biomedical Job Opportunities

Tools for Statistics

Free Software pDRAW32 to Draw DNA Analysis Charts

Biological Educational Resources

Genetics Education

Resources of Medical Biochemistry

Textbooks and Lab Manuals

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